H2N2V1, Main, Exploration, bibRecord, 002102

Cold-adapted vaccine strains of influenza a virus act as dominant negative mutants in mixed infections with wild-type influenza a virus

Identifieur interne : 002102 ( Main/Exploration ); précédent : 002101; suivant : 002103

Cold-adapted vaccine strains of influenza a virus act as dominant negative mutants in mixed infections with wild-type influenza a virus

Auteurs : Patricia Whitaker-Dowling [États-Unis] ; William Lucas [États-Unis] ; Julius S. Youngner [États-Unis]

Source :

Virology [ 0042-6822 ] ; 1990.

RBID : ISTEX:A777AB86EC7F78591BBAC691F4DEDF5C6067FF70

English descriptors

Teeft :
- Academic press, Assay, Biological properties, Brian murphy, Calf serum, Desirable trait, Double infection, Embryonated, Embryonated eggs, Fowl plague virus, Functional inactivation, Gene expression, Genes encoding, Infection, Influenza, Influenza virus, Influenza viruses, Interference phenomenon, Maassab, Mdck, Mdck cells, Monolayers, Mutant, Mutant viruses, Negative strand viruses, Noble agar, Nonpermissive temperature, Parental, Parental virus, Parental viruses, Phenotype, Plaque, Plaque assay, Protein synthesis, Reassortant, Reassortant virus, Reassortants, Replication, Vaccine, Vaccine strains, Viral, Viral genes, Viral plaque assay, Viral protein synthesis, Virus, Virus stocks, Virus vaccine, Youngner.

Abstract

Abstract: The cold-adapted reassortant of influenza A, which is a candidate live virus vaccine, interfered with the replication of parental wild-type virus in mixed infections of either MDCK cells or embryonated eggs. The interference occurred at either the permissive or nonpermissive temperature for the cold-adapted virus. In doubly infected cells, the yield of the wild-type virus was reduced by as much as 3000-fold and the protein synthesis phenotype expressed was that of the cold-adapted virus. The interference was detected even when infection with wild-type virus was carried out at a 9-fold excess or 2 hr before infection with the cold-adapted virus. As well as interfering with its wild-type parental virus, the cold-adapted virus also inhibited the replication of a heterologous influenza A subtype. In addition to its immunogenic potential, the ability to interfere with the replication of wild-type viruses is a desirable trait for any live, attenuated virus vaccine.

Url:

https://api.istex.fr/ark:/67375/6H6-ZMHC6N67-M/fulltext.pdf

DOI: 10.1016/0042-6822(90)90420-V

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: The cold-adapted reassortant of influenza A, which is a candidate live virus vaccine, interfered with the replication of parental wild-type virus in mixed infections of either MDCK cells or embryonated eggs. The interference occurred at either the permissive or nonpermissive temperature for the cold-adapted virus. In doubly infected cells, the yield of the wild-type virus was reduced by as much as 3000-fold and the protein synthesis phenotype expressed was that of the cold-adapted virus. The interference was detected even when infection with wild-type virus was carried out at a 9-fold excess or 2 hr before infection with the cold-adapted virus. As well as interfering with its wild-type parental virus, the cold-adapted virus also inhibited the replication of a heterologous influenza A subtype. In addition to its immunogenic potential, the ability to interfere with the replication of wild-type viruses is a desirable trait for any live, attenuated virus vaccine.</div>
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Cold-adapted vaccine strains of influenza a virus act as dominant negative mutants in mixed infections with wild-type influenza a virus

Cold-adapted vaccine strains of influenza a virus act as dominant negative mutants in mixed infections with wild-type influenza a virus

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri